DAVID 분석 예시
GOTERM_BP_
1에서 5로 내려갈수록 1에 포함되는 process이지만 더 자세한 process라고 할 수 있다.
* Ref.2의 '9. Why are there different levels for GO Annotation?' 참고
* BP_ALL: 1~5 결과 총합으로 P-value는 유지되지만 P-value를 adjust하는 pathway set수가 달라지기 때문에 adj-P value는 변동이 있음.
GOTERM_BP_FAT
GO FAT filters out very broad GO terms based on a measured specificity of each term (not level-specificity) and this way reduces the dependency between the terms.
GOTERM_BP_Direct
New GO category (GO Direct) provides GO mappings directly annotated by the source database (no parent terms included)
The Fold Enrichment is defined as the ratio of the two proportions. For example, if 40/400 (i.e. 10%) of your input genes involved in "kinase activity" and the background information is 300/30000 genes (i.e. 1%) associating with "kinase activity", roughly 10% / 1% = 10 fold enrichment.
[Reference]
[1] https://david.ncifcrf.gov/content.jsp?file=release.html
[2] https://david.ncifcrf.gov/content.jsp?file=FAQs.html
[3] https://david.ncifcrf.gov/helps/functional_annotation.html#summary